A systematic bi-genomic split-GFP assay illuminates the mitochondrial matrix proteome and protein targeting routes
Yury S Bykov, Solene Zuttion, Dunya Edilbi, Marina Polozova, Johanna Arnold, Sergey Malitsky, Maxim Itkin, Bruno Senger, Ofir Klein, Yeynit Asraf, Hadar Meyer, Hubert D Becker, Roza Kucharczyk, Maya Schuldiner
The paper uses a bi-genomic split-GFP assay to identify mitochondrial matrix and inner membrane proteins, revealing 50 previously unvisualized proteins and characterizing dually localized proteins.
bioRxiv · Apr 17, 2024Cell Biology