Exploiting HSD17B11-dependent dialkynylcarbinols cytotoxicity for facile CRISPR/Cas9-based gene inactivation
Bastien Dumais, Madeleine Bossaert, Patrick Seigneur, Alexandrine Rozié, Sophie Gasmi, Luce Fontanié--Roger, Maëlle Caroff, Valérie Maraval, Vania Bernardes‐Génisson, Dennis Gómez, Philippe Frit, Stéphanie Ballereau, Yves Génisson, Sébastien Britton
The paper demonstrates that inactivating HSD17B11 confers resistance to PAC, enabling efficient CRISPR/Cas9-based gene inactivation through PAC selection.
bioRxiv · Sep 20, 2026 · PreprintMolecular Biology