A highly stable engineered disulfide bond in the dimer interface of E. coli orotate phosphoribosyl transferase measures cytosolic redox conditions in yeast
Vilius Kurauskas, Sten Albrechtsen, Sarah K. Gersing, Kristoffer E. Johansson, Rasmus Hartmann‐Petersen, Martin Willemoës, Jakob Rahr Winther
The paper introduces a stable disulfide bond in E. coli OPRT to measure cytosolic redox potential in yeast, finding it to be -300 mV with enhanced stability in glutathione reductase mutants.
Biochemistry · Aug 6, 2025Peer-reviewedBiochemistry