Kristoffer E. Johansson

AF-CALVADOS uses AlphaFold to simulate multi-domain proteins at the proteome level, showing good performance across ordered and disordered regions.

This paper maps the functional effects of Parkin variants in mitophagy, distinguishing pathogenic from benign variants with high accuracy.

The paper identifies BAG6 and RNF126 as key players in PQC degradation of non-native missense protein variants using CRISPR screens and VAMP-seq.

The paper introduces a stable disulfide bond in E. coli OPRT to measure cytosolic redox potential in yeast, finding it to be -300 mV with enhanced stability in glutathione reductase mutants.

This study maps degrons in human transcription factors, identifies new degrons, and links degron potency to variant effects and transcriptional activation.

The paper systematically identifies degrons in human cytosolic proteins and links them to disease mechanisms through machine learning and structural analysis.

The paper identifies 5 stabilizing mutations for PYR1 variants using GMMA that increase thermal stability without affecting functionality across different ligand-binding capabilities.

The paper uses deep mutational scanning to show that protein degradation and toxicity are tightly correlated in ASPA variants linked to Canavans disease.

The paper develops QCDPred, a predictor of quality-control degrons in yeast proteins, showing that degrons are enriched in hydrophobic amino acids and depleted in negatively charged ones.

The paper screens split fluorescent protein variants to identify substitutions that enhance binding affinity.