Sequential Proofreading Enhances Specificity and Potency of CRISPR-CasX-based Epigenetic Repressors
Emeric J. Charles, Ross White, Ryan V. Tran, Amanda Mok, Lea Alice Kiefer, Kirsten A. Reimer, Ashley T Wong, Sterling Ripley-Phipps, Natalie Goh, T.C. Stevenson Keller, Carlos Santamaría, Christopher Duncan-Lewis, Beatriz Alvarado, Heather M. Kozy, Zoi Kyrkou, , Jason D. Fernandes
This paper introduces ELXRs, CRISPR-CasX-based epigenetic repressors that use sequential proofreading to enhance specificity and potency by reading chromatin state before DNA methylation.
bioRxiv · Aug 31, 2026 · PreprintMolecular Biology