Tailoring a CRISPR/Cas-based Epi-editor for Decreased Cytotoxicity and Enzymatic Specificity
Jacob Goell, Jing Li, Barun Mahata, Alex J Ma, Sunghwan Kim, Spencer T. Shah, Shriya Shah, Maria Contreras, Suchir Misra, Daniel Reed, Guy C. Bedford, Mario Escobar, Isaac B. Hilton
This paper engineers modified p300 variants fused to dCas9 to reduce cytotoxicity and enhance enzymatic specificity in CRISPR-based epi-editing.
bioRxiv · Sep 22, 2024 · PreprintSynthetic Biology