Isaac B. Hilton

Design of synthetic activators for programmable gene expression

This study designs synthetic activators for programmable gene expression by engineering coactivator-binding proteins and demonstrates their enhanced transcriptional activation capabilities.

Induction of hysteretic chromatin states by CRISPRa reveals locus-specific encoding of transcriptional memory

The paper uses CRISPRa to show that transcriptional memory can be induced in MSCs through locus-specific activation, linked to H3K27me3 loss and increased transcription factor reacquisition.

Loss of Syndecan-3 Drives a Pro-inflammatory, EndMT-prone Phenotype in Aortic Valve Endothelial Cells

This study identifies syndecan-3 as a regulator of inflammation and EndMT in aortic valve endothelial cells, showing its role in suppressing NF-kappaB activation and mesenchymal transition.

Predicting the effect of CRISPR-Cas9-based epigenome editing

The paper trains machine learning models to predict gene expression from histone PTMs using ENCODE data and validates predictions with CRISPR-Cas9-based epigenome editing experiments.

Robust fluorescent labeling and tracking of endogenous non-repetitive genomic loci

This paper develops a method to fluorescently label and track non-repetitive genomic loci in live human cells, showing gene-specific chromatin dynamics independent of transcription.

Tailoring a CRISPR/Cas-based Epi-editor for Decreased Cytotoxicity and Enzymatic Specificity

This paper engineers modified p300 variants fused to dCas9 to reduce cytotoxicity and enhance enzymatic specificity in CRISPR-based epi-editing.

Temporal analysis suggests a reciprocal relationship between 3D chromatin structure and transcription

The paper analyzes temporal changes in chromatin structure and gene expression during macrophage activation, finding a reciprocal relationship between loops and transcription.

Transgenic mice for in vivo epigenome editing with CRISPR-based systems

This paper describes the generation of two transgenic mouse lines for in vivo epigenome editing using CRISPR-based systems to activate or repress gene expression.