Author

T. J. Kleist

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 1 Cell Biology· 1 Plant Biology· 1

Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 3 Approach:experimental assay· 3 Assay:fluorescence / live-cell microscopy· 2 Organism:yeast· 2 Organism:Arabidopsis· 1 Research Focus:cell-cell communication· 1 Research Focus:dimensionality reduction· 1 Approach:genetic perturbation / CRISPR· 1

Frequent Co-Authors

M. M. Wudick· 3 shared W. B. Frommer· 3 shared M. Sadoine· 2 shared A. B. Hamacher· 1 shared E. S. Haswell· 1 shared E. Sunal· 1 shared F. Atanjaoui· 1 shared G. Maksaev· 1 shared

Activity by Year

2020-2023 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 29 mutual co-author links

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3 shared papersBioengineering

M. M. Wudick

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Author profile →

3 papers

Recent·Most cited

A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes shift fluorescent protein

J. O. Ejike, M. Sadoine, Y. Shen, Y. Ishikawa, E. Sunal, S. Hänsch, A. B. Hamacher, W. B. Frommer, M. M. Wudick, R. E. Campbell, T. J. Kleist

The paper introduces a new fluorescent protein, LSSmApple, and uses it to create a genetically encoded calcium sensor for yeast imaging.

bioRxiv · Jul 16, 2023Bioengineering

OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels

T. J. Kleist, I. W. Lin, S. Xu, G. Maksaev, M. Sadoine, E. S. Haswell, W. B. Frommer, M. M. Wudick

This paper describes the engineering of osmolality sensors called OzTracs by fusing fluorescent proteins to mechanosensitive ion channels, showing they report osmolality changes through indirect mechanisms.

bioRxiv · Feb 10, 2022Cell Biology

Sponging of glutamate at the outer plasma membrane surface reveals roles for glutamate in development

V. Castro-Rodriguez, T. J. Kleist, N. M. Gappel, F. Atanjaoui, S. Okumoto, M. Machado, T. Denyer, M. C. P. Timmermans, W. B. Frommer, M. M. Wudick

The paper shows that surface-displayed glutamate sensors affect plant development by sequestering glutamate at the plasma membrane, suggesting its role in signaling during growth and differentiation.

bioRxiv · Mar 24, 2020Plant Biology