Compartmentalized CRISPR Reactions (CCR) for High-Throughput Screening of Guide RNA Potency and Specificity
T. Supakar, A. Herring-Nicholas, E. A. Josephs
This paper introduces CCR, a method to screen gRNA potency and specificity in vitro by confining CRISPR reactions in microemulsions, enabling high-throughput validation of large gRNA variant libraries.
bioRxiv · May 7, 2024Bioengineering