U. Krengel

Calcium binding site in AA10 LPMO from Vibrio cholerae suggests modulating effects during environment survival and infection

The paper identifies a calcium-binding site in GbpA from V. cholerae that influences protein stability and substrate binding during environmental survival and infection.

Using Vibrio natriegens for high-yield production of challenging expression targets and for protein deuteration

This paper demonstrates that Vibrio natriegens produces challenging proteins and enables deuteration at higher yields than Escherichia coli.

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate

This paper investigates how the LPMO GbpA binds to chitin, showing it spreads on fibers and forms clumps, aiding bacterial microcolony formation.

Novel exported bifunctional fusion enzymes with chorismate mutase and cyclohexadienyl dehydratase activity: shikimate pathway enzymes teamed up in no man's land

The paper identifies rare exported bifunctional fusion enzymes combining chorismate mutase and cyclohexadienyl dehydratase in non-pathogenic bacteria, showing no catalytic benefit from fusion and suggesting a signaling role.

What drives chorismate mutase to top performance? Insights from a combined in silico and in vitro study

The paper investigates how the Mycobacterium tuberculosis chorismate mutase achieves high catalytic efficiency through structural and dynamic changes, both in silico and in vitro.

Insight into glycosphingolipid crypticity: Crystal structure of the anti-tumor antibody 14F7 and recognition of NeuGc GM3 ganglioside

This paper presents the crystal structure of the 14F7 antibody bound to NeuGc GM3 and shows how membrane composition and curvature influence its binding.

Crystal structures reveal that Lewis-x and fucose bind to secondary cholera toxin binding site - in contrast to fucosyl-GM1

The paper reports crystal structures and SPR data showing Lewis-x and fucose bind to a secondary cholera toxin binding site, distinct from the GM1 binding site.

Hypothesis: Hypoxia induces de novo synthesis of NeuGc gangliosides in humans through CMAH domain substitute

The paper investigates how hypoxia induces NeuGc GM3 ganglioside synthesis in human cells through candidate proteins identified via SILAC proteome analysis.

Towards new cholera prophylactics and treatment: Crystal structures of bacterial enterotoxins in complex with GM1 mimics

This paper presents crystal structures of bacterial enterotoxins complexed with GM1 mimics, revealing molecular interactions that could guide the design of new cholera prophylactics.