Simulation-guided sloppy DNA probe design for mismatch-tolerant hybridization
P. Bugga, V. Asthana, R. A. Drezek
This paper introduces a toehold-based exchange probe that detects nucleic acid sequences with multiple SNPs by incorporating controlled mismatches, showing effectiveness even with up to four mismatches and thermodynamic deviations.
bioRxiv · Dec 20, 2022 · PreprintBioengineering