Single-molecule analysis of purified proteins and nuclear extracts: insights from 8-oxoguanine glycosylase 1
M. Schaich, T. M. Weaver, V. Roginskaya, B. D. Freudenthal, B. Van Houten
This study compares single-molecule DNA binding dynamics of purified OGG1 and nuclear extracts using SMADNE, revealing differences in undamaged DNA binding and increased binding lifetime for a catalytically dead variant in nuclear extracts.
bioRxiv · Nov 1, 2023Biochemistry