Directed evolution of L-DOPA sensing and production enables efficient incorporation of an expanded genetic code into proteins
Andrew R. Gilmour, Qiyao Wei, Chad Wang, Jessica Hellinger, Prithvijeet Singh Kharnal, Vidya Pandarinath, Simon d’Oelsnitz, Eli Powell, Jennifer S. Brodbelt, Andrew D. Ellington, Ross T Thyer
This paper develops a L-DOPA sensing system and improves biosynthesis via directed evolution, achieving high-efficiency incorporation into proteins.
bioRxiv · Jun 26, 2025 · PreprintSynthetic Biology