Hua Ye

This study investigates the role of flpL gene in Aeromonas hydrophila pathogenicity, finding that its deletion reduces virulence by impairing biofilm formation and downregulating virulence genes, while increasing swimming and adhesion.

The paper describes a method to construct large DNA plasmids using yeast homologous recombination and CRISPR-Cas9 to remove a yeast replication element.

This study evaluates PacBio sequencing for direct, full-length resolution of complex DNA sequences, finding it effective for high GC, poly, and repeat sequences like rAAV without pre-fragmentation.