Deepening imaging-based spatial proteomics at high spatial resolution through controlled tissue resizing and in-situ bottom-up mass spectrometry
Kunyue Deng, Lingpeng Zhan, Yi Li, Jingpeng Zhang, Mingchuan Tang, Tianyi Chang, Gang Ji, Xinyang Shao, Xiang Xu, Xiaoyun Wei, Tianming Zhang, Jiacheng Yao, Jianbin Wang, Guanbo Wang, Yanyi Huang
The paper introduces IMPACT, a method to enhance spatial proteomics resolution and sensitivity through tissue compression and optimized digestion, demonstrating improved protein detection and anatomical resolution.
bioRxiv · Jun 12, 2025 · PreprintBiophysics