Enhancing Recombinant Vector Assembly Efficiency: A Novel Methodological Approach
Yuan Shu, Hailong Jiang, Haiyan Wang, Mengmeng Fu, Juan Wang, Zhen Liu, Yang Li
This paper introduces a method to improve recombinant vector assembly efficiency using high-temperature treatment followed by cooling, enhancing PCR product-vector connection.
bioRxiv · Aug 1, 2026 · PreprintBiochemistry