Fernando Bellido Molías

The paper benchmarks splicing predictors on synthetic gene variants to assess their performance and generalization beyond natural genomes.

The paper identifies missplicing as a common issue in heterologous gene expression and develops a splice-aware optimization algorithm to improve transgene performance.

This study shows that crispant founder mice can be used for phenotypic assessment despite genetic mosaicism, as observed null phenotypes were present in up to 100% of founders.