Synthetic BiologybioRxivPreprint
Expanding the CRISPR toolbox by engineering Cas12a orthologs of metagenomic discovery
Dagang Tao, Bingrong Xu, Sheng Li…·Shengsong Xie
The paper identifies and characterizes new Cas12a orthologs from metagenomic data, leading to variants with improved PAM recognition and editing efficiency for CRISPR applications.