Author

Gundra Sivakrishna Rao

King Abdullah University of Science and TechnologyORCID 0000-0001-5305-2320Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Bioengineering· 4

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:human· 3 Research Focus:variant calling· 3 Research Focus:drug response / drug discovery· 2 Research Focus:gene regulatory network inference· 2 Assay:CRISPR screen· 1 Approach:deep learning· 1 Assay:DNA methylation· 1

Frequent Co-Authors

Ahmed Saleh· 4 shared Magdy M. MahfouziD· 4 shared Ahmed M. KazlakiD· 3 shared Qiaochu WangiD· 3 shared Maazallah MasoodiD· 2 shared Rashid Abdi AmaniD· 2 shared Ahmed GhouneimyiD· 1 shared Ali H. A. ElbeheryiD· 1 shared

Activity by Year

2025-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 36 mutual co-author links

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4 shared papersBioengineering

Ahmed Saleh

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

BioengineeringbioRxivPreprintAug 21, 2026

Compact type II-D Cas9 nucleases for efficient and specific genome editing

Qiaochu Wang, Ahmed Saleh, Gundra Sivakrishna Rao…·Magdy M. Mahfouz

The paper identifies and characterizes compact Cas9d orthologs with high genome editing efficiency and specificity, suitable for AAV-based therapeutic applications.

DOI 10.64898/2026.08.17.745286

humanwhole-genome sequencing

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BioengineeringbioRxivPreprintAug 20, 2026

Compact type II-C Cas9 nucleases with expanded PAM access and high fidelity for therapeutic genome editing

Qiaochu Wang, Gundra Sivakrishna Rao, Rashid Abdi Aman…·Magdy M. Mahfouz

The paper identifies and characterizes three compact type II-C Cas9 orthologs with improved PAM compatibility and high fidelity for genome editing in human cells.

DOI 10.64898/2026.08.17.745178

humanproteomics

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BioengineeringbioRxivPreprintSep 16, 2025

Characterization and engineering of highly efficient Cas12j genome editors

Gundra Sivakrishna Rao, Wenjun Jiang, Mustapha Aouida…·Magdy M. Mahfouz

This study identifies and engineers novel Cas12j orthologues into efficient genome editors with enhanced editing activity and specific sequence requirements.

DOI 10.1101/2025.09.16.676532

humanCRISPR screen

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BioengineeringACS OmegaPeer-reviewedMay 14, 2025

Fusions of catalytically inactive RusA to FokI nuclease coupled with PNA enable programable site-specific double-stranded DNA breaks

Ahmed Saleh, Gundra Sivakrishna Rao, Qiaochu Wang, Magdy M. Mahfouz

The paper develops a new programmable site-specific nuclease system, PC-FIRA, that enables precise double-strand DNA breaks with high specificity and efficiency.

DOI 10.1021/acsomega.4c11282

DNA methylationexperimental assay

𝕏 PostBluesky