Daniel G. Olson

This study identifies substrates that C. thermocellum can transport and metabolize, revealing insights into its redox balance and fermentation product formation for biofuel production.

This study demonstrates that E. coli BL21 LysY/Iq can rapidly express and purify pyruvate decarboxylase from Zymomonas mobilis in 2 hours with yields comparable to traditional 16-hour methods at lower temperatures.

The paper identifies thermodynamic limitations in engineered microbial strains that prevent high-titer ethanol production by analyzing metabolite profiles and thermodynamic driving forces.

This paper engineers Clostridium thermocellum to produce 2,3-butanediol from cellulose, achieving a titer of 19.7 mM with a metabolic yield of 24%.

This paper identifies novel antibiotic resistance markers functional in thermophilic bacteria, with rbpA providing the highest selection range for genetic engineering.

The paper extends the KETCHUP tool to parameterize kinetic models using time-course data from cell-free systems, demonstrating accurate simulation of multi-enzyme systems.

This study uses cryo-EM and molecular dynamics to characterize AdhE ultrastructures in C. thermocellum, revealing a containment strategy for toxic intermediates in ethanol synthesis.