This paper introduces a deep learning method called AUTO-FLI for 3D fluorescence lifetime imaging in deep tissues, showing it can accurately reconstruct intensity and lifetime data in scattering media.

Journal of Biophotonics · Sep 1, 2026Peer-reviewedBioengineering

Real-Time Wide-Field Fluorescence Lifetime Imaging via Single-Snapshot Acquisition for Biomedical Applications

Vikas Pandey, Euan Millar, İsmail Erbaş, Luis Chavez, Jack Radford, Isaiah Crosbourne, Mansa Madhusudan, Gregor G. Taylor, Nanxue Yuan, Claudio E. Bruschini, Stefan T. Radev, Margarida M. Barroso, Andrew B. Tobin, Xavier Michalet, Edoardo Charbon A, , Xavier Intes

This paper introduces a real-time fluorescence lifetime imaging method using single-snapshot acquisitions, demonstrating its application in biomedical scenarios from microscopic to macroscale.

PhotoniX · Dec 19, 2025Peer-reviewedBioengineering

Depth-Resolved Macroscopic Fluorescence Lifetime Imaging via High-Spatial-Frequency Structured Illumination

Nanxue Yuan, Saif Ragab, Navid Ibtehaj Nizam, Vikas Pandey, Amit K. Verma, Tynan Young, John C. Williams, Margarida Barroso, Xavier Intes

This paper introduces HSF-FLI, a method to isolate subsurface fluorescence signals in MFLI, validated in vivo and ex vivo with mouse tumor models.

bioRxiv · Jun 10, 2025 · PreprintBioengineering