Xavier Michalet

This paper introduces a real-time fluorescence lifetime imaging method using single-snapshot acquisitions, demonstrating its application in biomedical scenarios from microscopic to macroscale.

AlliGator is a Windows open source software for analyzing fluorescence lifetime imaging data, offering FRET analysis and lifetime-derived quantity mapping.

This paper introduces a fluorescence lifetime-based method to measure membrane potential in Bacillus subtilis, achieving single-cell resolution and quantification of MP changes under chemical perturbations.