This paper develops and applies TR-LSCI for depth-sensitive, high-speed CBF imaging during ICP perturbations, revealing phase-dependent autoregulation transitions.

Journal of Biomedical Optics · Aug 28, 2026Peer-reviewedNeuroscience

Fluorescence-lifetime optical electrophysiology in contracting cardiomyocytes

Euan Millar, Eline Huethorst, Vytautas Žičkus, Giedre Astrauskaite, Jiuxuan Zhao, Gregor G. Taylor, Claudio E. Bruschini, Edoardo Charbon A, Godfrey L. Smith, Caroline Müllenbroich, Daniele Faccio

This paper introduces a method to measure cardiac electrophysiology using fluorescence lifetime imaging, revealing detailed voltage and calcium dynamics in cardiomyocytes.

Proceedings of the National Academy of Sciences · Jun 3, 2026Peer-reviewedBioengineering

Real-Time Wide-Field Fluorescence Lifetime Imaging via Single-Snapshot Acquisition for Biomedical Applications

Vikas Pandey, Euan Millar, İsmail Erbaş, Luis Chavez, Jack Radford, Isaiah Crosbourne, Mansa Madhusudan, Gregor G. Taylor, Nanxue Yuan, Claudio E. Bruschini, Stefan T. Radev, Margarida M. Barroso, Andrew B. Tobin, Xavier Michalet, Edoardo Charbon A, , Xavier Intes

This paper introduces a real-time fluorescence lifetime imaging method using single-snapshot acquisitions, demonstrating its application in biomedical scenarios from microscopic to macroscale.

PhotoniX · Dec 19, 2025Peer-reviewedBioengineering

Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera

Nathan Ronceray, Salim Bennani, Marianna Fanouria Mitsioni, Nicole Siegel, María J. Marcaida, Claudio E. Bruschini, Edoardo Charbon A, Rahul Roy, Matteo Dal Peraro, Guillermo P. Acuna, Aleksandra Rađenović

This paper develops a high-throughput single-molecule FLIM technique using a time-gated single-photon camera, achieving precise lifetime measurements with spatial multiplexing of over 3000 molecules.

Light Science & Applications · Aug 4, 2025Peer-reviewedBiophysics