This study characterizes the activity and role of Aedes aegypti chymotrypsin in blood digestion, showing its substrate specificity and the presence of a protease network, with EcR regulating its expression.

Scientific Reports · Mar 20, 2025Peer-reviewedBiochemistry

Comprehensive proteolytic profiling of Aedes aegypti mosquito midgut extracts: Unraveling the blood meal protein digestion system

Anthony John O’Donoghue, Chenxi Lui, Carter J. Simington, Saira Montermoso, Elizabeth Moreno-Galvez, Mateus Sá Magalhães Serafim, Olive E. Burata, Rachael M. Lucero, James ThienToan Nguyen, Daniel Fong, Khanh Tran, Neomi Millan, Jamie M. Gallimore, Kamille Parungao, Jonathan Fong, , Alberto A. Rascón

This study identifies midgut proteases in Aedes aegypti mosquitoes and their role in blood meal digestion using MSP-MS, revealing shifts in proteolytic activity and substrate specificity.

PLoS neglected tropical diseases · Feb 6, 2025Peer-reviewedBiochemistry

Characterization of essential eggshell proteins from Aedes aegypti mosquitoes

Carter J. Simington, Max E. Oscherwitz, Alyssa J. Peterson, Alberto A. Rascón, Brooke B. Massani, Roger L. Miesfeld, Jun Isoe

The paper identifies and characterizes essential eggshell proteins in Aedes aegypti mosquitoes, showing their roles in melanization and viability, but finds their RNAi phenotypes differ from EOF1 RNAi effects.

bioRxiv · Apr 6, 2020 · PreprintDevelopmental Biology