Peptide-Induced Formation of Extracellular Vesicles that are DistinctFrom Endogenous E. coli OMVs, and Provide an Enhanced Platformfor Protein Production and Purification.
Bree R. Streather, Tara A. Eastwood, Karen Baker, Mingzhi Liang, Alexandra Elizabeth Bailie, Tijn T. van der Velden, Lars J. C. Jeuken, Stanley W. Botchway, Lin Wang, Daniel P. Mulvihill
This paper describes a method using a Vesicle Nucleating Peptide (VNp) to produce recombinant extracellular vesicles in E. coli that are distinct from natural OMVs, enabling efficient protein production and purification.
bioRxiv · Mar 12, 2026 · PreprintBioengineering