Daniel P. Mulvihill

This paper describes a method using a Vesicle Nucleating Peptide (VNp) to produce recombinant extracellular vesicles in E. coli that are distinct from natural OMVs, enabling efficient protein production and purification.

This paper describes a high-throughput protocol for expressing, exporting, and assaying recombinant proteins from E. coli in a single multiwell plate, enabling direct use in enzymatic assays without purification.

This paper describes a method for producing functional monoclonal antibodies and fragments from E. coli, making antibody production more accessible and cost-effective.

The paper describes a protocol for producing and analyzing recombinant proteins packaged in membrane-bound vesicles from E. coli, enabling efficient downstream processing of challenging proteins.

This paper describes a system for producing functional recombinant proteins in extracellular vesicles from E. coli, achieving high yields and enabling continuous isolation.

The paper investigates how TORC2-dependent phosphorylation of myosin-1 in fission yeast affects calcium regulation and cell growth during mitotic and meiotic cycles.