Bree R. Streather

This paper describes a method using a Vesicle Nucleating Peptide (VNp) to produce recombinant extracellular vesicles in E. coli that are distinct from natural OMVs, enabling efficient protein production and purification.

The paper describes a protocol for producing and analyzing recombinant proteins packaged in membrane-bound vesicles from E. coli, enabling efficient downstream processing of challenging proteins.

This paper describes a system for producing functional recombinant proteins in extracellular vesicles from E. coli, achieving high yields and enabling continuous isolation.