Shifu Chen

This study validates high concordance between ddPCR and NGS for EGFR mutation detection across diverse biospecimens and shows pre-capture NGS libraries can substitute original DNA.

ECCFP is a bioinformatic pipeline for detecting eccDNA from long-read sequencing data, showing reduced false positives and higher sensitivity than existing methods.

CaMutQC is an R package that performs quality control and filtering of cancer somatic mutations, improving accuracy and comparability across studies.

The paper introduces a toolset for rapid identification of SARS-CoV-2 and other microorganisms from sequencing data, achieving 100% sensitivity and specificity for SARS-CoV-2 detection.

gencore removes duplicates and suppresses sequencing errors in NGS data, handling UMIs and generating detailed reports for quality control.

fastp unifies FASTQ quality control, adapter trimming, filtering, and base correction in one scan; single-threaded, it ran 2–5 times faster than FASTQC, Cutadapt, or Trimmomatic.