Chandran Pfitzner

This study shows that shortening the 4T poly-T tract in gRNA scaffolds improves transcript levels and editing efficiency, especially under low vector conditions, for both SpCas9 and SaCas9.

This study shows that animals can be engineered to express microbial enzymes to convert methylmercury into volatile mercury, reducing its toxicity and accumulation.

This study develops a rapid method for generating and screening transgenic black soldier flies using piggyBac mRNA and donor plasmids, achieving stable transgenic lines with 30-33% transformation efficiency.

This study investigates CRISPR/Cas9-based gene drives in mice, finding that robust Cas9 expression during meiosis is critical for efficient gene drive generation.