G.I. Godahewa

Enhancing gRNA Transcript levels by Reducing the Scaffold Poly-T Tract for Optimal SpCas9- and SaCas9-mediated Gene Editing

This study shows that shortening the 4T poly-T tract in gRNA scaffolds improves transcript levels and editing efficiency, especially under low vector conditions, for both SpCas9 and SaCas9.

Leveraging a natural murine meiotic drive to suppress invasive populations

This paper proposes a gene drive strategy called tCRISPR to suppress invasive mouse populations by leveraging the t haplotype's biased transmission and inactivating mutations in a female fertility gene.

Optimized nickase- and nuclease-based prime editing in human and mouse cells

This paper develops PEA1 plasmids and PE-nuclease systems for efficient prime editing in human and mouse cells, achieving high editing efficiency in some cell lines and fetal mice.