Author

Ashleigh Geiger

Commonwealth Scientific and Industrial Research OrganisationORCID 0000-0002-0203-1682Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Molecular Biology· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:CRISPR screen· 1 Organism:human· 1 Organism:mouse· 1 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

Caleb LushingtoniD· 2 shared Fatwa AdikusumaiD· 2 shared G.I. GodahewaiD· 2 shared Jayshen ArudkumariD· 2 shared Luke GierusiD· 2 shared Paul Quinton ThomasiD· 2 shared Yu Chinn Joshua CheyiD· 2 shared Chandran PfitzneriD· 1 shared

Activity by Year

2021-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 43 mutual co-author links

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2 shared papersMolecular Biology

Caleb LushingtonORCID

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2 papers

Recent·Most cited

Enhancing gRNA Transcript levels by Reducing the Scaffold Poly-T Tract for Optimal SpCas9- and SaCas9-mediated Gene Editing

Yu Chinn Joshua Chey, Luke Gierus, Caleb Lushington, Jayshen Arudkumar, Ashleigh Geiger, Lachlan G. Staker, Louise J. Robertson, Chandran Pfitzner, Jesse G. Kennedy, Ryan H. B. Lee, G.I. Godahewa, Paul Quinton Thomas, Fatwa Adikusuma

This study shows that shortening the 4T poly-T tract in gRNA scaffolds improves transcript levels and editing efficiency, especially under low vector conditions, for both SpCas9 and SaCas9.

BMC Genomics · Feb 12, 2025Peer-reviewedMolecular Biology

Optimized nickase- and nuclease-based prime editing in human and mouse cells

Fatwa Adikusuma, Caleb Lushington, Jayshen Arudkumar, G.I. Godahewa, Yu Chinn Joshua Chey, Luke Gierus, Sandra Piltz, Ashleigh Geiger, Yatish Jain, Daniel Reti, Laurence O W Wilson, Denis C. Bauer, Paul Quinton Thomas

This paper develops PEA1 plasmids and PE-nuclease systems for efficient prime editing in human and mouse cells, achieving high editing efficiency in some cell lines and fetal mice.

Nucleic Acids Research · Sep 16, 2021Peer-reviewedMolecular Biology