Javier Santos‐Moreno

The paper investigates the role of periplasmic helices and cytoplasmic domains of PulL and PulM in the assembly of the Klebsiella type II secretion system, showing their critical involvement in protein secretion and pseudopilus formation.

The paper constructs synthetic genotype networks of gene regulatory networks in E. coli to study robustness and evolvability in genotype-phenotype maps.

This paper designs and demonstrates CRISPRi-based synthetic gene circuits in E. coli, including an oscillator, bistable switch, and stripe pattern-forming IFFL, showing the potential of CRISPRi for dynamic and multistable circuit construction.

The paper introduces a modular cloning framework for assembling synthetic gene circuits in E. coli, reducing construction time and costs through a two-step Gibson assembly process.