Hadiastri Kusumawardhani

This paper engineers a multi-step differentiation program in E. coli to generate self-organized spatial patterns through symmetry breaking, paracrine, and autocrine signaling.

This paper develops a method for intercellular communication in E. coli using M13 phagemids and CRISPR-based gene regulation to build logic gates for multicellular computing.

The paper shows that using the same guide RNA structure for CRISPRi and CRISPRa in E. coli prevents context-dependent effects, ensuring predictable gene circuit behavior.

The paper constructs synthetic genotype networks of gene regulatory networks in E. coli to study robustness and evolvability in genotype-phenotype maps.