Xiaoyi Li

The paper introduces OPTIMuS PRIME-Del, a CRISPR-based method for multiplexed, precise deletion of cis-regulatory elements, validated through molecular tagging and scRNA-seq analysis.

The paper studies the evolutionary changes of mammalian enhancers by analyzing orthologous sequences across multiple species and using deep learning to model functional changes.

The paper shows that inhibiting the HMWR complex causes mitotic defects and enhances cancer cell vulnerability when combined with genotoxic agents.

The paper develops monoclonal gastruloids from single mouse embryonic stem cells and uses DNA Typewriter to reveal heritable lineage biases in early development.

This paper introduces P3 editing, a method that links protein-protein proximity to CRISPR-Cas9 dual-component guide RNA formation, enabling genome editing triggered by molecular interactions in human cells.

This paper introduces Genome-Shuffle-seq, a method for generating and mapping structural variants in mammalian genomes, and demonstrates its application in mouse embryonic stem cells.

The paper designs and characterizes diverse genomic parts for precise genome editing and molecular recording in mammalian cells.

The paper describes a CRISPRa screening method combining multiplex perturbations with sc-RNA-seq to identify cell-type-specific regulatory elements and their target genes.

This paper identifies epigenetic features affecting prime editing efficiency and shows how chromatin state can be manipulated to enhance or reduce editing efficiency at specific genomic loci.

This study develops a genetic tool to reversibly inhibit miRNA-mediated gene repression in vivo and shows tissue-specific dependencies on miRNA function for homeostasis and regeneration.

This paper introduces a method to identify miRNA targets in vivo using Halo-Enhanced Ago2 Pulldown and demonstrates its application in mouse models.