Structure-Guided Loop Grafting Improves Expression and Stability of Influenza Neuraminidase for Vaccine Development
Pramila Rijal, Leiyan Wei, Guido C. Paesen, David Ian Stuart, Mark R. Howarth, Kuan-Ying Arthur Huang, Thomas A. Bowden, Alain Townsend
This paper improves the expression and stability of influenza neuraminidase by grafting antigenic loops from low-expressing variants onto high-expressing scaffolds, resulting in vaccine-effective hybrid proteins.
eLife · Sep 9, 2025Peer-reviewedImmunology