Pierfrancesco Pagella

This paper develops an HNSCC-on-a-chip model with tunable engineered extracellular matrices, vasculature, and innervation to study tumor behavior and therapeutic responses.

This study shows Wnt/β-catenin signaling can establish new regulatory elements by remodeling chromatin, beyond its traditional role as a molecular switch.

The paper maps transcription factor binding across mouse tissues at different developmental stages, revealing tissue-specific activity and conserved regulatory regions with functional relevance in humans.

This study shows that Nogo-A deletion affects trigeminal ganglion neurons and tooth innervation, altering synapse function and neurotrophin signaling.

This study shows that Wnt signaling changes CTCF binding and chromatin structure by creating new CTCF binding sites that depend on β-catenin.

This study identifies TBX3 as a functional component of the Wnt/β-catenin complex in human colorectal cancer, showing it modulates metastasis-related transcription through direct protein interactions.

ICEBERG identifies genome-wide binding events of transcriptional regulators by using extensive CUT&RUN replicates, revealing previously undetectable events in colorectal cancer.

The paper examines single-cell Wnt signaling responses in human embryonic stem cells and finds that Wnt target gene activation varies among cells, showing uncoupling in gene expression patterns.

The paper identifies CUT&RUN-specific problematic genomic regions, called blacklists, which persist despite standard quality control measures and improve peak calling reliability when removed.

This study shows that Notch signaling, specifically through Jagged1, influences tooth-crown shape variations in mice, mirroring evolutionary changes observed in Muridae.

The paper shows that β-catenin binds different genomic loci over time, leading to cell-type specific chromatin responses termed plastic or elastic during Wnt signaling.

The paper introduces a modified CUT&RUN protocol, LoV-U, to reliably profile β-catenin binding and identifies its tissue-specific target genes in human and mouse cells.