Author

Ryota Tomioka

Kyoto UniversityORCID 0009-0006-2022-9971Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Biochemistry· 1 Systems Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Assay:proteomics· 2 Organism:mouse· 1 Research Focus:protein folding & dynamics· 1

Frequent Co-Authors

Ayana TomiokaiD· 2 shared Kosuke OgataiD· 2 shared Yasushi IshihamaiD· 2 shared Eisuke KanaoiD· 1 shared Issei MoriiD· 1 shared Koshi ImamiiD· 1 shared Makoto AritaiD· 1 shared Yosuke IsobeiD· 1 shared

Activity by Year

2023-2025 · peak 1/yr

Co-Author Collaboration Network

8 top collaborators · 28 mutual co-author links

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2 shared papersBiochemistry

Ayana TomiokaORCID

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2 papers

Recent·Most cited

BiochemistryMolecular & Cellular ProteomicsPeer-reviewedNov 20, 2025

Bottom-up proteomics under acidic conditions using protease type XIII from Aspergillus saitoi

Ryota Tomioka, Ayana Tomioka, Kosuke Ogata, Yasushi Ishihama

This study demonstrates that protease type XIII from Aspergillus saitoi improves bottom-up proteomics under acidic conditions by enhancing sequence coverage and reducing modification artifacts compared to trypsin.

1 citation

proteomicsexperimental assay

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Systems BiologybioRxivPreprintJun 5, 2023

One thousand samples per day capillary-flow LC/MS/MS for high-speed, high-sensitivity and in-depth proteomics

Ayana Tomioka, Ryota Tomioka, Issei Mori…·Yasushi Ishihama

This paper develops a high-speed LC/MS/MS system achieving 1,000 samples per day with high sensitivity and depth, demonstrating its application in spatial proteomics of mouse brain.

7 citations

mouseproteomics

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