David A. Scott

This study shows that phosphorylation of α-synuclein at Serine-129 is a physiologic trigger for protein interactions and synaptic function, challenging its previous association with pathology.

The paper tests 16 Cpf1 orthologs for genome editing activity in eukaryotic cells, identifying four active enzymes including Mb3Cpf1 with robust indel formation and PAM tolerance.

The paper demonstrates multiplex gene editing in mammalian cells and mouse brains using a single CRISPR array processed by Cpf1 without requiring additional factors.

BLISS quantifies DNA double-strand breaks in situ with high sensitivity and efficiency, showing Cpf1 has higher specificity than Cas9.