Ruben Van Paemel

This study benchmarks DNA methylation deconvolution methods for tumoral fraction estimation in cfDNA, finding CelFiE most accurate and introducing DecoNFlow as a comprehensive pipeline.

The paper introduces cf-RRBS, a method for methylation profiling of fragmented plasma DNA, enabling cost-effective and accurate non-invasive lung cancer subtyping.

The paper evaluates RNA purification kits and blood collection tubes for exRNA quality, finding that preservation tubes and purification methods significantly affect RNA stability, sensitivity, and reproducibility.

This study evaluates how blood collection tubes and time delay affect cfDNA methylation, finding robust results across tubes but recommending EDTA tubes processed within 24 hours.

The paper evaluates SILVER-Seq data for DNA contamination and finds low exonic and splice read fractions, suggesting contamination issues in the technology.

This study demonstrates that reduced representation bisulfite sequencing of cell-free DNA can accurately classify pediatric solid tumors with high sensitivity.

The paper evaluates the SMARTer Stranded Total RNA-Seq method's performance in quantifying total RNA from various human biofluids and extracellular vesicles, finding it accurate, precise, and capable of detecting thousands of genes.