Crisandra Jade Diaz

Efficient secretion of a plastic degrading enzyme from the green algae Chlamydomonas reinhardtii

This paper demonstrates the efficient secretion of a plastic-degrading enzyme, PHL7, from the green algae Chlamydomonas reinhardtii, showing its ability to degrade PET and bio-based polyurethanes.

Sustainable production of plastic-degrading enzymes in Chlamydomonas pacifica

The paper demonstrates the production of a plastic-degrading enzyme, PHL7, in the extremophile alga Chlamydomonas pacifica in open raceway ponds.

Establishing the green algae Chlamydomonas incerta as a platform for recombinant protein production

This paper evaluates Chlamydomonas incerta as a platform for recombinant protein production, showing higher expression levels for mCherry and comparable performance for xylanase and PHL7 compared to C. reinhardtii.

Description of a novel extremophile green algae, Chlamydomonas pacifica, and its potential as a biotechnology host

This paper characterizes a new extremophile green algae, Chlamydomonas pacifica, highlighting its biotechnological potential for biofuel and biodegradable plastic production.

Engineering microalgal cell wall-anchored proteins using GP1 PPSPX motifs and releasing with intein-mediated fusion

This paper develops a system for anchoring and controlled release of proteins in Chlamydomonas reinhardtii using GP1 PPSPX motifs and pH-sensitive inteins.

Engineering the novel extremophile alga Chlamydomonas pacifica for high lipid and high starch production as a path to developing commercially relevant strains

This paper genetically engineers the extremophile alga Chlamydomonas pacifica to produce high lipid and starch levels for biodiesel and thermoplastic polyurethane precursors, demonstrating scalability in pilot-scale cultivation.

Mutational sources of trans-regulatory variation affecting gene expression in Saccharomyces cerevisiae

The paper identifies trans-regulatory mutations affecting gene expression in S. cerevisiae and shows their genomic distribution overlaps with segregating variants.