Stephen Patrick Mayfield

This paper demonstrates the efficient secretion of a plastic-degrading enzyme, PHL7, from the green algae Chlamydomonas reinhardtii, showing its ability to degrade PET and bio-based polyurethanes.

The paper identifies and overexpresses key transcription factors in Chlamydomonas pacifica to enhance lipid accumulation for biodiesel production.

The paper demonstrates the production of a plastic-degrading enzyme, PHL7, in the extremophile alga Chlamydomonas pacifica in open raceway ponds.

This study develops and validates low-cost, high-throughput screening methods for algae tolerance to extreme pH, temperature, salinity, and light using agar plates and liquid cultures.

This paper evaluates Chlamydomonas incerta as a platform for recombinant protein production, showing higher expression levels for mCherry and comparable performance for xylanase and PHL7 compared to C. reinhardtii.

This paper characterizes a new extremophile green algae, Chlamydomonas pacifica, highlighting its biotechnological potential for biofuel and biodegradable plastic production.

This paper develops a system for anchoring and controlled release of proteins in Chlamydomonas reinhardtii using GP1 PPSPX motifs and pH-sensitive inteins.

This paper genetically engineers the extremophile alga Chlamydomonas pacifica to produce high lipid and starch levels for biodiesel and thermoplastic polyurethane precursors, demonstrating scalability in pilot-scale cultivation.

The paper identifies cis-regulatory elements in algae to create synthetic promoters, validated through in vivo testing and high-throughput screening.

The paper develops a cell surface display system in Chlamydomonas reinhardtii by identifying functional anchoring points for protein expression on the cytoplasmic membrane and cell wall.