BioengineeringbioRxivPreprint
A Nanoheater-Integrated Fluorescence Lifetime Thermometer for Investigating Subcellular Heat Shock Factor 1 Responses
Hettimudalige Dilini Nisansala, Deshan Basnayake, Kayoko Nomura…·Satoshi Arai
This paper develops a nanoheater-thermometer to measure subcellular temperature and monitor HSF1 responses in living cells, showing that localized heating induces HSF1 foci at lower temperatures than whole-cell heating.