A Nanoheater-Integrated Fluorescence Lifetime Thermometer for Investigating Subcellular Heat Shock Factor 1 Responses
Hettimudalige Dilini Nisansala, Deshan Basnayake, Kayoko Nomura, Yohei Kono, Takeru Yamazaki, Yuya Matsuda, Cong Quang Vu, Takeshi Shimi, Satoshi Arai
This paper develops a nanoheater-thermometer to measure subcellular temperature and monitor HSF1 responses in living cells, showing that localized heating induces HSF1 foci at lower temperatures than whole-cell heating.
bioRxiv · Sep 3, 2026 · PreprintBioengineering