Amy S. Gladfelter

A phase oscillator model of cell cycles reveals nuclear density control in a branched fungal network

The paper models nuclear division in a branching fungal network to show that asynchronous cycles and density-responsive branching stabilize nuclear density.

Study of Protein-Protein Interactions in Septin Assembly: Multiple amphipathic helix domains cooperate in binding to the lipid membrane

This study uses molecular dynamics simulations to show how amphipathic helix domains cooperate in binding to lipid membranes, stabilizing septin structures.

Life history stage-dependent nuclear selection in chimeric fungi

The paper investigates nuclear selection in chimeric fungi during different life stages, finding strong selection during spore germination and mycelium establishment but not during mycelium growth.

Sequence and structure of protein binding sites in RNA impact biomolecular condensates

The paper investigates how RNA-protein binding site context, not just valence, affects biomolecular condensate properties in Ashbya gossypii.

RNA-specific local translation is patterned by condensates for multinucleate cell growth

The paper shows that Whi3 condensates regulate local translation in syncytial fungi, linking condensate composition and RNA features to spatially patterned translation during cell growth.

Lipid packing and local geometry influence septin curvature sensing

This study shows that lipid packing and membrane geometry jointly regulate septin localization in vitro and in cells.

Mechanisms of nuclear segregation in a multinucleate multibudding yeast

This study examines mitotic nuclear segregation in the multibudding yeast Aureobasidium pullulans, revealing distinct mechanisms compared to uninucleate yeasts.

Cooperativity in septin polymerization is tunable by ionic strength and membrane adsorption

This study investigates how ionic strength and membrane adsorption influence septin polymerization, showing salt-dependent cooperativity and context-dependent assembly modes.

Immiscible proteins compete for RNA binding to order condensate layers

The paper shows that core and shell proteins in paraspeckles compete for RNA binding, leading to layered condensate organization through immiscibility and competitive binding.

mRNA interactions promote cotranslational association of heteromeric membrane proteins

The paper shows that mRNA interactions promote cotranslational association of heteromeric hERG1a/1b ion channels, with structural diversity and mRNA interactions influencing translation modes.

Optimized vectors for genetic engineering of Aureobasidium pullulans

The paper designs and tests 25 optimized vectors for genetic engineering of Aureobasidium pullulans, enabling dual protein expression and homology-based gene modification.

Charge distribution and helical content tune the binding of septins amphipathic helix domain to lipid membranes

This study investigates how charge distribution and helical content of amphipathic helix domains affect septin binding to lipid membranes using simulations and experiments.

RNA promotes synapsin phase separation providing a platform for local translation

The paper shows RNA promotes synapsin phase separation, forming condensates that organize synaptic vesicles and enhance local translation.

Negative feedback equalizes polarity sites in a multi-budding yeast

The paper identifies Pak1 as a key protein mediating negative feedback in yeast to enable multiple polarity sites and budding.

Biomolecular condensates control and are defined by RNA-RNA interactions that arise in viral replication

The paper investigates how RNA-RNA interactions in viral replication are controlled by biomolecular condensates, showing that sequence complementarity and condensate properties influence RNA organization.

RNA encodes physical information

The paper develops a genetic algorithm to study how synonymous mutations affect mRNA structure and reveals a physical code in the genetic code that influences RNA-protein complex properties.

A single septin from a polyextremotolerant yeast recapitulates many canonical functions of septin hetero-oligomers.

The paper biochemically characterizes KpAspE, a Group 5 septin from Knufia petricola, showing it recapitulates functions of canonical septin hetero-octamers in vitro.

Comparative analysis of the syncytiotrophoblast in placenta tissue and trophoblast organoids using snRNA sequencing

This study compares syncytiotrophoblast gene expression in placental tissue and trophoblast organoids using snRNA-seq, identifying distinct nuclear subtypes and environmental influences on STB gene expression.

Double-stranded RNA drives SARS-CoV-2 nucleocapsid protein to undergo phase separation at specific temperatures

This study shows that SARS-CoV-2 N-protein uses two distinct RNA motifs, both requiring dsRNA, to drive phase separation at specific temperatures, enabling multiple viral functions.

Guanidine hydrochloride reactivates an ancient septin hetero-oligomer assembly pathway in budding yeast

The paper shows that guanidine hydrochloride reactivates an ancient septin hexamer assembly pathway in yeast by mimicking a missing arginine sidechain in Cdc3.