D. Dafydd Jones

This paper genetically encodes 3-cyano-L-tyrosine into a fluorescent protein, altering its chromophore isomerization state and spectral properties.

The paper shows dipolar relaxation in the protein matrix during ESPT in GFP, which influences the reaction pathway and is on the same timescale as ESPT.

The paper describes the engineering of mCoral, a modified mCherry variant with improved hydrogen peroxide tolerance and pH responsiveness, and elucidates its structural and dynamic properties.

The paper engineers a brighter and more photostable variant of avGFP by modifying chromophore interactions, resulting in improved fluorescence and resistance to photobleaching.

This paper engineers a green fluorescent protein that changes its chromophore charge state through copper-dependent homodimerisation, resulting in a 30-fold fluorescence emission ratio change.