Single-stranded HDR templates with truncated Cas12a binding sequences improve knock-in efficiencies in primary human T cells
Ana-Maria Nitulescu, Weijie Du, Viktor Glaser, Jonas Kath, Eric J. Aird, Grégoire Cullot, Robert Greensmith, Nanna S. Mikkelsen, Maik Stein, Rasmus Otkjær Bak, Michael M. Kaminski, Jacob E. Corn, Dimitrios L. Wagner
This study improves CRISPR-Cas12a knock-in efficiency in human T cells by using single-stranded DNA templates with double-stranded Cas-target-sequences, achieving up to 90% knock-in rates for transgene inserts.
Molecular Therapy — Nucleic Acids · May 19, 2025Peer-reviewedImmunology