Himadri B. Pakrasi

The paper investigates the nitrogenase gene cluster in Cyanothece to identify essential genes for nitrogen fixation in phototrophs and provides a blueprint for synthetic nitrogen-fixing systems.

NEKO is an AI workflow using LLMs to extract and organize scientific knowledge from literature, providing more specific and actionable insights than existing models.

This paper streamlines the genome of Synechococcus elongatus UTEX 2973 to improve growth and productivity using CRISPR/Cas3 and computational tools.

The paper develops a reversibly induced CRISPRi system in Synechocystis sp. PCC 6803 to knock down and recover expression of the psbD gene, demonstrating its effectiveness in modulating Photosystem II activity.

This paper engineers nitrogen fixation activity in a non-diazotrophic cyanobacterium by transferring nitrogenase genes and improving O2 tolerance, achieving over 30% N2 fixation activity.