This paper develops a portable biosensor for rapid ASFV detection in whole blood using microfluidic paper-based devices, achieving high sensitivity and specificity without DNA extraction.

Biosensors and Bioelectronics · Oct 1, 2026Peer-reviewedBioengineering

ThermiQuant(TM) MegaScan: High-throughput isothermal reactor with quantitative colorimetric readout for paper-based nucleic acid amplification tests

Bibek Raut, Gopal Palla, Virendra Kumar, Andrew Fleck, Bilal Ahmed, Josiah Davidson, Ryan F. Relich, Jon P. Schoonmaker, J. Alex Pasternak, Mohit S. Verma

npj Biosensing · May 12, 2026Peer-reviewedBioengineering

Rapid High-Throughput Analysis of Bovine Skeletal Muscle Fiber Morphology via Automated Fluorescent Microscopy and MuscleBos software

Hamood Rehman, Kyrstin M. Gouveia, Rebecca K. Coombe, Jacquelyn P. Boerman, J. Alex Pasternak, James F. Markworth

Meat and Muscle Biology · Apr 24, 2026Peer-reviewedPhysiology

A solid-state heater-imager for quantitative evaluation of colorimetric isothermal nucleic acid amplification on paper

Bibek Raut, Gopal Palla, Dan Nguyen, Rachel A. Munds, Abdullah Bayram, Virendra Kumar, Bilal Ahmed, Cindy Mayorga, Aaron C. Ault, Aaron Gilbertie, J. Alex Pasternak, Mohit S. Verma

The paper introduces a dual-sided heater-imager system for quantitative colorimetric isothermal nucleic acid amplification on paper, achieving temperature uniformity and real-time analysis.

Lab on a Chip · Jan 1, 2026Peer-reviewedBioengineering

Defining Cellular Diversity at the Swine Maternal-Fetal Interface Using Spatial Transcriptomics and Organoids

Cole R. McCutcheon, Allyson Caldwell, Liheng Yang, Elisa Crisci, J. Alex Pasternak, Carolyn B. Coyne

This study uses spatial transcriptomics and organoids to define cellular diversity at the swine maternal-fetal interface, revealing conserved trophoblast populations and novel markers.

PLoS Biology · Aug 28, 2025Peer-reviewedDevelopmental Biology