Mohit S. Verma

A rapid, field-deployable paper-based biosensor for the detection of African swine fever virus in whole blood

This paper develops a portable biosensor for rapid ASFV detection in whole blood using microfluidic paper-based devices, achieving high sensitivity and specificity without DNA extraction.

Bacteroidales on Harvesters: Baseline Prevalence and Abundance

The paper assesses fecal contamination on harvester surfaces using qPCR and develops a portable LAMP assay for rapid hygiene assessment.

ThermiQuant™ AquaStream: A portable instrument for quantitative colorimetric isothermal nucleic acid amplification reactions in paper and tube formats

ThermiQuant AquaStream is a portable device that enables real-time colorimetric LAMP assays in both microPADs and tubes with high sensitivity and precision.

ThermiQuant(TM) MegaScan: High-throughput isothermal reactor with quantitative colorimetric readout for paper-based nucleic acid amplification tests

Detection of five viruses commonly implicated with Bovine Respiratory Disease using loop-mediated isothermal amplification

The paper develops qLAMP and RT-qLAMP assays for detecting five BRD-associated viruses with high sensitivity and specificity, and introduces a streamlined pipeline for assay development.

A solid-state heater-imager for quantitative evaluation of colorimetric isothermal nucleic acid amplification on paper

The paper introduces a dual-sided heater-imager system for quantitative colorimetric isothermal nucleic acid amplification on paper, achieving temperature uniformity and real-time analysis.

Development and Evaluation of Novel Quantitative PCR (qPCR) and Loop-Mediated Isothermal Amplification (LAMP) Assays for Bovine Adenovirus Type 7

The paper develops and evaluates qPCR and qLAMP assays for detecting BAV-7, achieving high specificity and agreement, with qLAMP showing potential for field use.

Mechanistic Evaluation of Amplification Lag in Paper-Based Colorimetric Loop Mediated Isothermal Amplification (LAMP) and Its Reduction by BSA Pre-Coating

The paper evaluates amplification lag in paper-based LAMP assays and shows BSA pre-coating reduces it by improving diffusion and reducing adsorption.

Real-time monitoring of attenuated cytomegalovirus using Raman spectroscopy allows non-destructive characterization during flow

This paper develops a Raman spectroscopy tool for real-time monitoring of attenuated CMV in flow, demonstrating its ability to detect viral particles at high flow rates and across concentrations.

Design and development of a field-deployable water bath for loop-mediated isothermal amplification assay

The paper develops a field-deployable water bath, IsoHeat, for LAMP reactions, which maintains target temperatures and operates efficiently in various environmental conditions.

Limit of detection of Raman spectroscopy using polystyrene particles from 25 to 1000 nm in aqueous suspensions

This study determines the limit of detection of Raman spectroscopy for polystyrene particles in aqueous suspensions, showing its potential for detecting small particles like viruses.

A Paper-based Loop-Mediated Isothermal Amplification (LAMP) Assay for Highly Pathogenic Avian Influenza

This paper develops a paper-based LAMP assay for detecting H5 subtype avian influenza virus with a detection limit of 500 copies per reaction, offering a low-cost, user-friendly diagnostic tool for resource-limited settings.

Propidium monoazide is unreliable for quantitative live-dead molecular assays

This study shows that propidium monoazide (PMA) is unreliable for quantitative viability assays when bacterial concentration and composition are unknown, but suitable for qualitative assessment with known dead microbes or extracellular DNA.