Christopher J. Cotter

This study identifies DNA repair mechanisms that influence CRISPR-Cas antifungal efficacy in Candida albicans and shows how to tune Cas9 lethality through repair template availability and enzyme activity.

This study identifies cellular stress tolerance as a key factor in genetic transformability of Candida species and develops a method to improve CRISPR screening efficiency.

CRISPR-GRIT enables precise multiplexed genome editing in C. albicans by integrating repair templates into guide RNAs, overcoming the need for co-transformation.

This study identifies how Yarrowia lipolytica metabolizes hydrocarbons and DP oil, revealing proteome reallocation for growth and chemical production, but also growth inhibition due to reduced protein biosynthesis.